By C.O. Chichesters, E.M. Mrak, G.F. Stewart (Eds.)
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Additional resources for Advances in Food Research, Vol. 26
Levine, D. , and Cooney, C. L. 1973. Isolation and characterization of a thermotolerant methanol utilizing yeast. Appl. Microbiol. 26, 982-990. Lindbolm. M. 1974. The influence of alkali and heat treatment on yeast protein. Biotech. Bioengr. 16, 1495. , and Morgan H. 1974. Enzymatic RNA reduction in disintegrated cells of S . cerevisiae. Biotech. Bioengr. 16, 1123-1 133. , and Vitols, E. 1962. A simple high-speed blender for the disintegration of microorganisms. Biochem. Biophys. Acta. 59, 231.
1973). Thus, it appears that the requirement for energy for cellular maintenance places an exceedingly high demand on the use of the carbon energy source at elevated temperatures; this in turn leads to an inefficient conversion of the carbon source to SCP. To conclude this section on the problem of heat production, it is useful to compare the most commonly considered substrates for SCP production in terms 22 C. L. COONEY ET AL. 08. 04 0 30 35 40 TEMPERATURE 45 50 (*C) FIG. 10. Specific growth rate of isolate DL-I as a function of temperature as determined by chemostat washout (Levine and Cooney, 1973).
Therefore, for the concentration and purification process to be most efficient, it would be rational first to consider the following questions: 1. Which components are valuable or potentially valuable? 2. In what order are these components important? 3 . Which components should be eliminated? 4. What are the desirable characteristics of the components? 5. What processes preserve or enhance these characteristics? 6. , 1970), equivalent generally to 20 gm of yeast. If SCP is to be used as a major ingredient in food, removal of nucleic acids is the essential first step.