By Pierce D. A.
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Extra info for Age-time patterns of cancer to be anticipated from exposure to general mutagens
SK-4100, Vector), and for alkaline phosphatase: Fast Red TR/Naphtol AS-MX (Sigma, Cat. #. B5655). ● Hematoxylin (Vector, Cat. # H-3401). Staining jars (Sigma, Cat. # S5641). 1. Permount (Fisher Cat. # SP15-100). 7. 8. Equipment Immunohistochemistry of Pancreatic Neoplasia 2. Coverslips (Fisher Cat. # 12-548-5P). ● 56°C incubator for baking slides. ● IHC jar. ● PAP Pen (Vector, Cat. # H-4000). ● Humidifying chamber for incubation of slides. ● Microtome for cutting 3–5 μM thick sections. ● Microwave or pressure cooker or water bath (depending upon the protocol used for antigen retrieval).
Arch Pathol Lab Med 131:18–43 Maitra A, Adsay NV, Argani P, IacobuzioDonahue C, De Marzo A, Cameron JL, Yeo CJ, Hruban RH (2003) Multicomponent analysis of the pancreatic adenocarcinoma progression model using a pancreatic intraepithelial neoplasia tissue microarray. Mod Pathol 16:902–912 Sauter G, Simon R, Hillan K (2003) Tissue microarrays in drug discovery. Nat Rev Drug Discov 2:962–972 Schoenberg Fejzo M, Slamon DJ (2001) Frozen tumor tissue microarray technology for analysis of tumor RNA, DNA, and proteins.
Although the depth of the recipient core can be set by the depth stop, there is no depth stop that will set the depth of the donor core. This depth varies when using different donor blocks, since the tissue blocks are of varying thickness, which determines the limit of the depth at which the cores are taken. For an experienced technologist the depth of the donor core is modified based on the thickness of the donor block. A separate “Depth Stop Kit” can be purchased from Beecher that can be used to obtain uniform lengths of donor cores; this kit may be useful if all donor blocks are of similar thickness.